Oil-in-Water Nanosized Emulsions for Drug Delivery and Targeting. Tamilvanan Shunmugaperumal

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СКАЧАТЬ and R₂ (showing negative regression coefficient values). The interaction effects between A and B was favorable for response R₁ (showing a positive regression coefficient value of 13.75) and non‐favorable for R₂ and R₃ (showing negative regression coefficient values). However, it was observed that the interaction between A and C had an inverse effect for all three responses variables studied. Noticeably, quadratic effects (A², B², and C²) were also seen between all three independent variables and all of the studied three response variables (MPS, PDI, and ZP or R₁, R₂, and R₃). The highest and positive quadratic effect for all three independent variables was noticed for the MPS response variable R₁ (showing a positive regression coefficient value of 15.35). Similarly, the highest and negative quadratic effect was also noted for the MPS response variable R₁ (showing a negative regression coefficient −118.30).

TABLE 2.8. Estimated Coded and Actual Model Equations Along with ANOVA of Regression Coefficients for Face‐Centered Central Composite Design (CCD) Effects (Main or Linear, Quadratic and Interaction) Against the Critical Quality Attributes (CQAs, Dependent Variables) to Determine the Best Fitted Quadratic Equation

CQAs	Model Equations	R 2	Adjusted R2
Mean particle size (R1)	Coded: R1= 569.86 + 65.20 × A + 25.21 × B + 43.03 × C + 13.75 × AB − 55.18 × AC − 10.38 × BC + 15.35 × A2 − 118.30 × B2 − 91.00 × C2					0.8186	0.6553
	Actual: PS = −5992.56500 + 663.59545 × a + 88.29447 × b + 120.25931 × c + 4.58333 × a × b − 8.82800 × a × c − 0.138333 × b × c + 61.41818 × a2 − 3.28598 × b2 − 0.582371 × c2
Polydispersity index (R2)	Coded: R2 = 0.6038 + 0.0325 × A + 0.0234 × B + 0.0960 × C − 0.0106 × AB − 0.0649 × AC − 0.0494 × BC + 0.0180 × A2 + 0.0075 × B2 + 0.1455 × C2					0.7188	0.4658
	Actual: PDI = −9.27017 + 0.800295 × a + 0.061847 × b +0.194161 × c −0.003542 × a × b − 0.010380 × a × c − 0.000658 × b × c + 0.071818 × a2 + 0.000207 × b2 − 0.000931 × c2
Zeta potential (R3)	Coded: R3 = 28.89 + 0.0500 × A + 1.32 × B − 2.76 × C − 2.21 × AB − 0.9125 × AC + 2.06 × BC − 0.8136 × A2 − 1.56 × B2 − 4.26 × C2					0.6525	0.3397
	Actual: ZP = −180.64250 + 31.48864 × a − 0.037576 × b + 4.44347 × c − 0.737500 × a × b − 0.146000 × a × c + 0.027500 × b × c − 3.25455 × a2 − 0.043434 × b2 − 0.027287 × c2
CCD effects	Variables	R 1		R 2		R 3
		F‐value	P‐value	F‐value	P‐value	F‐value	P‐value
Linear term	A	7.61	0.0202	1.09	0.3219	0.0013	0.9714
	B	1.14	0.3111	0.5629	0.4704	0.9391	0.3554
	C	3.32	0.0986	9.47	0.0117	4.11	0.0702
Interaction term	AB	0.2709	0.6141	0.0928	0.7668	2.11	0.1769
	AC	4.36	0.0633	3.46	0.0924	0.3590	0.5624
	BC	0.1542	0.7028	2.01	0.1872	1.83	0.2054
Quadratic term	A 2	0.1161	0.7403	0.0911	0.7689	0.0981	0.7605
	B 2	6.89	0.0254	0.0157	0.9027	0.3624	0.5606
	C 2	4.08	0.0711	5.99	0.0344	2.69	0.1317

      Note: A/a: Castor oil, B/b: Chitosan, C/c: Poloxamer.

      The coefficient significance of the quadratic СКАЧАТЬ