Chemical Analysis. Francis Rouessac
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Название: Chemical Analysis
Автор: Francis Rouessac
Издательство: John Wiley & Sons Limited
Жанр: Химия
isbn: 9781119701347
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To recommend the best method in order to resolve an analytical problem, there is a science called chemometrics. Its purpose is to help the analyst, as a function of specific requirements and aims: minimum sampling plan, appropriate methodology, data processing and interpretation of results. Thanks to the use of IT tools, it seeks to provide a correct answer by exploiting the results with statistical methods in order to reduce the number of tests for long or costly analyses.
In this way, users may acquire devices that meet the standard of precision and quality necessary to acquire certification or to have a laboratory officially recognized for the quality of its results. These accreditation procedures are now imposed by many control organizations around the world.
Analytical chemistry is therefore essential in many fields other than the traditional ones of chemistry or chemical engineering. It can be found in fields as varied as medicine, food, biochemistry, the environment (pollution), safety (explosives, drugs and chemicals), and art. Now more widely used in human activities than ever, analytical chemistry can be of benefit to us all.
Chapter 1 General Aspects of Chromatography
INTRODUCTION
Chromatography, the process by which the components of a mixture can be separated, has become one of the primary analytical methods for the identification and quantification of compounds. The basic principle is founded on the concentration equilibrium of the components of interest between two immiscible phases. One is called the stationary phase, because it is immobilized within a column or fixed upon a support, while the second, called the mobile phase, is forced through the first. The phases are chosen such that the components of the sample have differing solubilities in each phase. The differential migration of compounds leads to their separation. This hydrodynamic process, which has been constantly evolving since its discovery, is an analytical method that no laboratory involved in molecular analysis can ignore, as its applications are so numerous.
Objectives
Review the principle of chromatography
Distinguish between separation and analysis by chromatography
Explain the protocol of a chromatographic analysis
Use a chromatogram and model its signals
Describe the various retention parameters
Discuss the hydrodynamic aspects of a separation
Define parameters influencing the efficiency of a separation
Classify the various chromatography techniques
Describe the steps of an assay using chromatography
1.1 GENERAL CONCEPTS OF ANALYTICAL CHROMATOGRAPHY
Chromatography is a physico‐chemical method of separation of components within mixtures, liquid or gaseous, in the same vein as distillation, crystallization or fractionated extraction. The applications of this process are therefore potentially numerous, since many heterogeneous mixtures, or those in solid form, can be dissolved by a suitable solvent (which becomes, of course, a supplementary component of the mixture).
A basic chromatographic process may be described as (Figure 1.1):
1 A vertical, hollow glass tube (the column) is filled with a suitable finely powdered solid, the stationary phase.
2 At the top of this column is placed a small volume of the sample to be separated into individual components.
3 The sample is then forced through the column from inlet to outlet by continuous addition of the mobile phase, carrying the various constituents of the sample along with it. If the components migrate at different velocities, they will become separated from each other and can be recovered, each in solution with the mobile phase.
While this use of chromatography has continued since its origins, this process became a method of analysis with the idea of measuring the retention time of compounds through the column in order to identify them. To do so, it became essential to control certain parameters (flow rate, temperature, etc.) and a detector had to be placed at the column’s outlet to identify compositional changes in the mobile phase. This form of chromatography, whose goal is not simply to recover the components but to measure their retention time, has developed slowly.
The identification of a compound by chromatography is achieved by comparison. To identify a compound, which may be either A or B, using chromatography, we compare its retention time with those for the two reference compounds A and B previously recorded using the same apparatus and the same experimental conditions.
Figure 1.1 A basic experiment in chromatography. (a) The necessary ingredients (C, column; SP, stationary phase; MP, mobile phase and sample); (b) introduction of the sample; (c) start of elution; (d) recovery of the products following separation.
In this experiment, there was no true separation (A and B were pure products), only a comparison of the products’ retention times. However, this method does have three weaknesses: the procedure is fairly slow; absolute identification is unattainable; and the physical contact between the sample and the stationary phase could modify the sample’s properties, in particular the retention times.
This specific method of separation, in its modern form, was first undertaken at the beginning of the twentieth century by the botanist Mikhail Tswett (or Tsvet), who is credited with inventing the terms chromatography and chromatogram.
The technique has improved considerably since its beginnings. Nowadays, chromatographs are piloted by software programs that run highly efficient miniature columns able to separate nano‐quantities of sample. These instruments comprise a complete range of accessories designed to ensure repeatability of successive experiments by the perfect control of the different parameters of separation. Thus it is possible to obtain, during successive analyses of the same sample conducted several hours apart, recordings that are reproducible to within a second (Figure 1.2).
The specific recording that is obtained for each separation is called a chromatogram. It corresponds to a two‐dimensional diagram that reveals the variations of composition of the eluting mobile phase as it exits the column. To obtain this read‐out, СКАЧАТЬ