Parasitology. Alan Gunn

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      The transmission mechanism(s) employed by the Sauroleishmania remain uncertain. Within the sandfly vector, these species tend to remain in the posterior regions of the gut, and therefore, it is unlikely that transmission occurs when the sandfly feeds. Furthermore, sandflies do not usually defaecate whilst feeding, so it is unlikely that transmission resembles that of T. cruzi by triatomid bugs. A third possibility is that the transmission occurs through the lizards consuming infected sandflies.

Much of the work on how Leishmania establishes and develops within its mammalian hosts involves mice as model organisms and those few Leishmania species capable of being cultured in the laboratory. One should therefore be careful of extrapolating from these studies to the likely behaviour of other Leishmania species and infections in other hosts. Mice and humans are both mammals, but one cannot assume that their immune systems react identically to the same infectious agent. Furthermore, leishmaniasis manifests itself in numerous ways. Therefore, different Leishmania species and strains probably exhibit variations in the way they establish themselves and interact with the host immune system. Nevertheless, all species follow a basic pattern of development following their entry into the blood stream that involves morphological and physiological transformations and establishment in the mononuclear phagocytes and in particular the macrophages.

      How Leishmania Establishes Within Mammalian Phagocytes

      After an infected sandfly feeds, mononuclear phagocytes quickly detect and ingest the promastigotes that enter the blood circulation. Initial attachment of a parasite to a phagocyte begins at the tip of the parasite’s flagellum via a ligand‐receptor process. The principal ligands are phosphoglycans and the zinc metalloprotease enzyme GP63 (glycoprotein 63) on the surface of the promastigote whilst on the phagocyte, a variety of complement receptors are involved in the attachment process. After phagocytosing the Leishmania parasite, a phagocyte holds it within a membrane‐bound vesicle called a phagosome. Lysomes then fuse with the phagosome and discharge hydrolytic enzymes and microbicidal peptides into it. They also acidify the contents. The structure then becomes to known as a parasitophorous vacuole or phagolysosome. Different species of Leishmania cause the formation of different types of phagolysosomes: those produced by L. mexicana tend to be large and contain many parasites whilst those formed by L. donovani tend to be much smaller.

      The combination of a rise in temperature associated with moving to a mammalian host and the drop in pH caused by enclosure within a phagolysosome induce the promastigote to change into the amastigote form. This transformation takes about 1–4 hours following ingestion and is essential if the parasite is to survive the acidic pH and hydrolytic enzymes within the phagolysosome. Transformation includes changes the composition of the cell surface phosphoglycans. Some of these, the glycoinositol phospholipids (GIPLs) directly inhibit the production of nitric oxide (NO) by the phagocyte. The expression of GP63 is downregulated in the amastigote but they continue to express it, and it is important for both the survival of the parasite and as a virulence factor (Olivier et al. 2012). The membrane changes associated with transformation to the amastigote stage means amastigotes invade phagocytes using a different set of ligands and receptors to the promastigote stage. Despite these changes, phagocytes remain capable of destroying amastigotes. Therefore, the reasons why some people develop serious or even fatal infections, whilst in others the infection is resolved, possibly without displaying any symptoms is uncertain.

      4.2.1.2 Visceral Leishmaniasis

      Classical visceral leishmaniasis is commonly known as kala‐azar and causes fevers like those of malaria. Both malaria and kala‐azar occur in the same areas, so it is important for doctors to distinguish between them. The name kala‐azar (black head) derives from the symptomatic darkening of the forehead and mouth of patients suffering from visceral leishmaniasis. In India, great plagues of visceral leishmaniasis occurred in Assam in the late nineteenth and early twentieth centuries that depopulated whole villages. Serious outbreaks still occur today, and visceral leishmaniasis remains an important cause of morbidity and mortality in over 70 countries around the world. Most cases of visceral leishmaniasis occur in South Asia (~67%), but it is also a big problem in parts of East Africa (e.g., Sudan in the region bordering with Ethiopia). There is also a focus of infection in South America, especially in Brazil.

The clinical picture of visceral leishmaniasis differs geographically. There is, nonetheless, a basic pattern to the course of the disease. The first stage begins when a papule develops at the site of the sandfly bite, but this eventually regresses. Low‐grade recurrent fevers then develop anything from 10 days to 2 years or more afterwards, and these persist throughout the course of the disease. Within the spleen, immune‐related responses destroy red blood cells, and this causes anaemia. In addition, the liver becomes enlarged (hepatomegaly) as does the spleen (splenomegaly). Enlargement of the spleen results from a combination of hyperplasia induced by the need to produce new mononuclear phagocytes and from infected mononuclear phagocytes filling with parasites. The patient often suffers from diarrhoea and this, together with the fever, leads to anorexia, malnutrition, and dehydration. If the disease is not treated, 90% of those suffering visceral leishmaniasis will die. Recovery can be rapid and complete with or without treatment. However, in many cases the parasite persists and may appear on the skin in raised macules causing the disfiguring condition post kala‐azar dermal leishmaniasis.

      4.2.1.3 Post Kala‐Azar Dermal Leishmaniasis

      Post kala‐azar dermal leishmaniasis (PKDL) is a condition that is usually associated with L. donovani and develops as sequel to visceral leishmaniasis in 2.5–20% of cases (hence ‘post‐kala‐azar’). It may manifest itself anything from immediately afterwards to several years following the condition. It is characterised by the development of nodules and/or macules that can be extensive and cover any area of the body and may be mistaken for leprosy. The nodules are irregular raised masses on the skin surface whilst macules (Latin macula = blemish or small spot) are flat discoloured areas on the skin surface. These regions contain numerous amastigotes, and if they occur on exposed parts of the body, they are a ready source of infection for sandflies. The identification and treatment of patients suffering from PKDL is therefore an important part of any control programme. There are marked differences the occurrence and development of PKDL between countries, which suggests that host, and/or parasite factors may be important in whether it develops. For example, most cases of PKDL (~50%) occur in the Sudan and the condition tends to develop more rapidly there than in India.

      Some workers consider that the development of PKDL is associated with the incomplete or inefficient treatment of visceral leishmaniasis following treatment with the drugs sodium stibogluconate and pentamidine. However, PKDL may also occur after treatment with miltefosine. In India, Das et al. (2009) found about 20% of PKDL cases occurred in people for whom there was no record of either visceral leishmaniasis or the prescription of the drugs used to treat it. However, these people tested positive for L. donovani and therefore carried an asymptomatic infection.

      4.2.1.4 Cutaneous Leishmaniasis

Cutaneous leishmaniasis manifests itself in a variety of different forms depending upon the species of Leishmania. Clinically, cutaneous leishmaniasis divides into three basic types depending upon how the disease presents. Localized Cutaneous Leishmaniasis (LCL) generally takes the form of a dry ulcer. It develops at the bite site of the sandfly vector and usually heals by itself although this may take some time and leave permanent scarring. This form of disease is common in India, Central Asia, the Middle East, and parts of southern Europe; L. major and L. tropica are responsible for most infections in these regions. LCL is also common in South America where species such as L. venezuelensis and L. mexicana are responsible. Diffuse (disseminated) cutaneous Leishmaniasis (DCL) is a rarer and much more serious condition than LCL. It manifests as numerous СКАЧАТЬ