Diagnostics and Therapy in Veterinary Dermatology. Группа авторов

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СКАЧАТЬ www.idexx.com VARL Liquid Gold VARL Liquid‐phase matrix enzyme immunometric system using avidin‐biotin interaction to capture allergen‐specific IgE combined with solid‐phase ELISA methodology to quantify IgE Serum Quantitative assay to measure allergen‐specific IgE www.varlallergy.com Molecular diagnostics for infectious organisms Test Company Sample required Information obtained Website Ringworm (Dermatophyte) RealPCR™ Panel IDEXX Hair, skin scrapings (from active border of lesion) Dermatophyte (Microsporum spp. or Trichophyton spp.) Positive results indicate infection when clinical lesions are present, but indicate nonclinical carriage state without clinical lesions. Negative results indicate no or few Microsporum spp. or Trichophyton sp. organisms in sample. Because positive PCR results can be due to active infection, fomite carriage or nonviable fungal organisms from a successfully treated infection, PCR for dermatophyte may be most useful for initial diagnosis, while fungal culture may be a better indicator of mycologic cure. Note: False negatives have been seen with this test, so culture is still recommended www.idexx.com Sarcoptes Antibody ELISA IDEXX Serum Measures presence of anti‐Sarcoptes antibodies in serum. Positive results indicate possible Sarcoptes infection, or false positive due to house dust mite hypersensitivity (these antigens cross react). Dogs may remain positive for up to 6 months after successful treatment. Negative results indicate lack of exposure, early infection, or immunosuppressive medication interactions. Sensitivity is 84.2%, and specificity is 89.5% http://www.idexx.com Tick PCR Panels Antech: Tick Borne PCR Panel Whole blood (EDTA) Indicates the presence of DNA from various tick‐borne organisms www.antechdiagnostics.com Cornell University College of Veterinary Medicine, Animal Health Diagnostic Center PCR Indicates the presence of DNA from Ehrlichia canis, Neorickkettsia risticii, Anaplasma phagocytophilum, Bartonella spp., hemotropic Mycoplasma spp. Many additional individual PCR tests available on website http://www.vet.cornell.edu/animal‐health‐diagnostic‐center/testing/protocols/blood‐parasite IDEXX: Tick/Vector Comprehensive RealPCR Panel Indicates the presence of DNA from Anaplasma spp., Babesia spp., Bartonella spp., canine hemotropic Mycoplasma, Ehrlichia spp., Hepatozoon spp., Leishmania spp., Neorickettsia risticii, and Rickettsia rickettsii. Quantifies DNA of Leishmania spp. www.idexx.com North Carolina State University Vector Borne Disease Diagnostic Laboratory Indicates the presence of DNA from Bartonella spp., Anaplasma spp., Ehrlichia spp., Babesia spp., Cytauxzoon felis, Mycoplasma spp., Leishmania spp., Rickettsia spp. http://cvm.ncsu.edu/research/labs/clinical‐sciences/vector‐borne‐disease Tick Serology Panels Antech: AccuPlex™ 4 Fastpanel PCR Canine Tick Borne Profile w/Lyme Serum Serum Whole blood (EDTA) Measures antibodies to Borrelia burgdorferi, Anaplasma sp., and Ehrilichia sp., and measures Dirofilaria immitis antigen Measures presence of DNA from several tick‐borne organisms, and measures Borrelia burgdorferi antibodies. Also offers B. burgdorferi PCR and Western Blot www.antechdiagnostics.com Cornell University College of Veterinary Medicine, Animal Health Diagnostic Center Serum Measures antibodies to Rickettsia rickettsii, Neorickettsia risticii, Ehrlichia canis, Brucella canis, Bartonella spp. by IFA; measures antibodies to Borrelia burgdorferi, Ehrlichia canis, and Anaplasma spp. by ELISA; measures Leishmania infantum by indirect ELISA http://www.vet.cornell.edu/animal‐health‐diagnostic‐center/testing/protocols/blood‐parasite IDEXX: Tick Panel – immunofluorescence antibody (IFA) test Lab 4Dx® Plus Test (ELISA) Lyme Quant C6^® Serum Serum Serum Urine Quantifies Ehrlichia canis, Rickettsia rickettsii, and Borrelia burgdorferi antibody Measures antibodies to Anaplasma phagocytophilum, Anaplasma platys, Ehrlichia canis, Ehrlichia ewingii, and Borrelia burgdorferi, and presence of Dirofilaria immitis antigen Measures Borrelia burgdorferi antibody, limited chemistry profile, limited complete blood count, SDMA, urinalysis, and urine protein:creatinine ratio. Lyme Quant C6 testing is available with the RealPCR panel. www.idexx.com North Carolina State University Vector Borne Disease Diagnostic Laboratory Serum Measures antibodies to Bartonella hensalae, Bartonella vinsonii, Bartonella koehlerae, Babesia canis, Babesia gibsonii, Ehrlichia canis, Rickettsia rickettsii, Leishmania infantum, Anaplasma sp., Borrelia burgdorferi http://cvm.ncsu.edu/research/labs/clinical‐sciences/vector‐borne‐disease Bacterial PCR Provided by many research institutions and universities. Polymerase chain reaction (PCR) tests are available for common dermatologically relevant bacteria, including Staphylococcus spp., Pseudomonas sp., Streptococcus sp., E. coli, Bordetella sp., Mycobacteria spp., Bartonella sp., etc. Refer СКАЧАТЬ